Reference genome sequences of two cultivated allotetraploid cottons, Gossypium hirsutum and Gossypium barbadense

Transformation of Gossypium hirsutum and Gossypium barbadense by Rhizobium rhizogenes: Hairy root induction and gossypol production

Gene Flow between Gossypium hirsutum L. and Gossypium barbadense L. is Asymmetric

As both Pima cotton (Gossypium barbadense L.) and Acala (Upland; G. hirsutum L.) are grown in the southwestern United States, understanding gene fl ow within and among these species is important to maintain genetic purity. In small scale and commercial fi elds, gene fl ow decreased exponentially from 1.44% at 1 m to less than 0.50% at 10 m in Acala cotton. Corresponding values for Pima cotton w...

Genetic and phenotypic effects of chromosome segments introgressed from Gossypium barbadense into Gossypium hirsutum

MBI9915 is an introgression cotton line with excellent fiber quality. It was obtained by advanced backcrossing and continuous inbreeding from an interspecific cross between the upland cotton (Gossypium hirsutum) cultivar CCRI36 as the recurrent parent and the sea island cotton (G. barbadense) cultivar Hai1, as the donor parent. To study the genetic effects of the introgressed chromosome segment...

A detailed RFLP map of cotton, Gossypium hirsutum x Gossypium barbadense: chromosome organization and evolution in a disomic polyploid genome.

We employ a detailed restriction fragment length polymorphism (RFLP) map to investigate chromosome organization and evolution in cotton, a disomic polyploid. About 46.2% of nuclear DNA probes detect RFLPs distinguishing Gossypium hirsutum and Gossypium barbadense; and 705 RFLP loci are assembled into 41 linkage groups and 4675 cM. The subgenomic origin (A vs. D) of most, and chromosomal identit...

High efficient multisites genome editing in allotetraploid cotton (Gossypium hirsutum) using CRISPR/Cas9 system

Gossypium hirsutum is an allotetraploid with a complex genome. Most genes have multiple copies that belong to At and Dt subgenomes. Sequence similarity is also very high between gene homologues. To efficiently achieve site/gene-specific mutation is quite needed. Due to its high efficiency and robustness, the CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 system has exer...